Background and intended use incucyte nuclight bacmam 3. Recombinant protein production in baculovirusinsect. This should include, the wiley titles, and the specific portion of the content you wish to reuse e. Search for user documents, sdss, vector maps and sequences. The bacmam virus expression system has gradually become one of the major. Two days after nrvm isolation, appropriate volume of celllight reagent 30 l1,000,000 cells was used as modi. A bispecific antibody promotes aggregation of ricin toxin on.
Citations are the number of other articles citing this article, calculated by crossref and updated daily. Nov 30, 2015 effect on recombinant pgp or abcg2 expression of treatment with sodium butyrate, tunicamycin, thaps, and baptaam. Purchase of this product does not convey a license to expand, amplify. By combining the h 2o 2selective properties of orp1 and rogfp, it is possible to selectively quantitate h 2o 2 in cells with the premo.
The bacmam virus expression system has gradually become one of the. Jul 19, 2019 we have labelled r3 cells with gfp expressing vector celllight nucleusgfp, bacmam 2. Fluorescence labeling and detection strategies in cellular. Including an unlabeled control, various gfpexpressing bacmam celllight reagents were added at 2% vv and incubated for 24 hr figures 1 and 2. These metrics are regularly updated to reflect usage leading up to the last few days. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level bsl 1 in most. Bacmam pgptransduced hela cells express functional pgp. Incucyte nuclight reagents incucyte nuclight bacmam 3. Article views are the countercompliant sum of full text article downloads since november 2008 both pdf and html across all institutions and individuals. Multiple mechanisms underlying acquired resistance to taxanes in selected docetaxelresistant mcf7 breast cancer cells.
Capz and actin capping dynamics increase in myocytes after a. Web to pdf convert any web pages to highquality pdf files. Simply culture cells, add virus, incubate, and visualize bacmam 2. Cell penetrating caspase substrates promote survival of the. In parallel, cells were plated into 6 separate dishes and labeled with histone 2b h2bgfp at concentrations of 10%, 5%, 2%, 1%, 0. For shrna knockdown, cells were infected with the lentivirus encoding shtopbp1 and shwee1 or control. If the contents are pure text, like a cv, the file size is usually 10kb per. Drug metabolism and disposition february 2016, 44 2 180188. Pharmacologic evaluation of these proteins in vitro remains a challenge. Cells are the basic units of life that can be used to understand various physical and biological processes in the body.
Pdf use of baculovirus bacmam vectors for expression of. Search for user documents, sdss, vector maps and sequences, application notes, formulations. Mutations in mct8 in patients with allanherndondudley. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level bsl 1 in most laboratories. Bacmam system for rapid recombinant protein expression in. Celllight reagents contain a signal peptide or protein fused to cfp. Intracellular antigen labeling and manipulation by antibodies have been longthought goals in the field of cell research and therapy. Detecting green fluorescent protein using the attune acoustic. Analyze the transduction efficiency the next day or freeze the cells for future use. Generation of ips cells using a bacmam multigene expression system article pdf available in cell structure and function 36 2.
It might not work with latest versions of openoffice. Jjx12 consists of rtad10, a camelid single variable domain vhh antibody directed against an epitope on ricins enzymatic subunit rta, linked via a 15mer peptide to rtbb7, a vhh. Click on the links above to find and download examples of aim pen and. In this study, human abc transporters were expressed in polarized epithelial cell monolayers transduced using the bacmam baculovirus gene transfer system. Gfp fluorescence peak fraction analysis based nanothermometer. Raw data from sds sequence detection system files were recorded and analyzed by the 2ct. Use pdf download to do whatever you like with pdf files on the web and regain control. Purchase of this product does not convey a license to expand, amplify, or otherwise. Improved baculovirus vectors for transduction and gene. Over 10 million scientific documents at your fingertips. Mutations in mct8 in patients with allanherndondudleysyndrome affecting its cellular distribution simone kersseboom, gertjan kremers, edith c. Phosphatidylinositol 4,5bisphosphate regulates capz 1 and actin dynamics in response to mechanical strain jieli li and brenda russell department of physiology and biophysics, university of illinois at chicago, chicago, illinois. Assessment of gfp expression and viability using the tali. We recommend fixed cell format for large sample sizes, such as for hcs.
Phosphatidylinositol 4,5bisphosphate regulates capz 1 and. Identify targets like a pro solutions for identifying early leads against gpcrs and ion channel targets our flipr tetra highthroughput cellular screening system is fast, reliable, and remarkably easy to configure. Bacmam technology overview thermo fisher scientific us. Taking advantages of small sizes and unique structures of the singledomain antibodies, here, we presented a novel approach to. Brough2, damon finnigan2, and kasia paczyna1 1mo l ecular dv is, now part ofm san alyt al t hn g s, sunnyv c li rnia u a 2glaxosmith kline, u. Pdf generation of ips cells using a bacmam multigene.
We offer a standard pdf file and a zip pdf file for you to download. When stored as described, the incucyte nuclight bacmam 3. Bacmam system to study abc transporters in polarized cells. Evaluation of cryopreserved bacmam transduced aequorin cells. Effect on recombinant pgp or abcg2 expression of treatment with sodium butyrate, tunicamycin, thaps, and baptaam. Infected nrvms are returned to the culture incubator for at least 16 h. The needle stub was quickly removed, creating a narrow 0. One of the aliquots was mixed with the peptide dl18 10. Bacmam technology is extremely well tolerated without apparent cytopathic effects, even at very high 1,000 virus particle to cell multiplicity of infection moi ratios. Initial titration of rat p2y1 bacmam in u 2 os cells. Detecting red fluorescent protein using the attune acoustic. Construction of a supramolecular drugdrug delivery system. Perform plaque assay to evaluate the virus titer of v0, v1, and v2 virus.
Baculovirus expression vector system bevs is an established. Evaluation of cryopreserved bacmam transduced aequorin cells in 384 and 1536well suspension cell assays using the aequorin option for the fliprtetra system carole crittenden1, parita shah2, stephen j. Each 1 ml aliquot of cells was stimulated by adding 100 ng il2 1 l of 0. Timelapse confocal imaging datasets to assess structural and. Four representative cell types were transduced u2os, 293msr, hekn, and chos.
Role of cd15 and cd15s in the cellular mechanisms of cancer. Optimization of gene delivery methods in xenopus laevis kidney a6. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning. System files were recorded and analyzed by the 2ct method using sds rq. For best results, transduce cells at a confluence of no more than 70%. The bacmam vectors generated in this study were based on two. Article plus supplemental information find, read and cite all the research you need on researchgate. Pdf multiple mechanisms underlying acquired resistance to. Pdf on nov 25, 2015, domenico iuso and others published document s2. For aortic banding a 4 0 silk suture was placed around the aorta between the right and left carotids and a 25g needle stub was tied in place. Presentation, storage and stability incucyte nuclight bacmam 3.
To monitor dynamics of a particular region by stroboscopic analysis of cell dynamics saced 15, the sequence files were imported into imagej and a particular region. Using the bacmam baculovirus system to study expression and. Jjx12 is an engineered bispecific antibody against ricin, a member of the medically important ab family of toxins that exploits retrograde transport as means to gain entry into the cytosol of target cells. Bacmam celllight reagents were added at 2% vv and incubated for 24 hr figures 1 and 2. In parallel, cells were plated into 6 separate dishes and labeled with histone 2b h2brfp at concentrations of 10%, 5%, 2%, 1%, 0. With the cloud connect utility, you can upload files by simply placing them in a folder on your. Rapid delivery of nanobodiesvhhs into living cells via.
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